4OSN
Crystal structure of engineered HCMV glycoprotein B Domain II
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 89.922, 89.922, 75.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.030 - 1.760 |
| R-factor | 0.20337 |
| Rwork | 0.202 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.375 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.030 | 1.810 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 18070 | |
| Completeness [%] | 98.0 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292.15 | 1.5M Lithium Sulfate, 0.1M Na-HEPES salt, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292.15K |
