Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2011-09-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11435 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 205.258, 205.258, 235.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 123.540 - 4.802 |
| R-factor | 0.2765 |
| Rwork | 0.276 |
| R-free | 0.28160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 200.000 | 4.970 |
| High resolution limit [Å] | 4.800 | 10.340 | 4.800 |
| Rmerge | 0.114 | 0.049 | 0.573 |
| Number of reflections | 45946 | ||
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 98.0 | 99.9 | 95.8 |
| Redundancy | 4.2 | 7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 75 mM Sodium Acetate, 1.0 M Ammonium Phosphate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
