4OPO
Crystal structure of P domain from norovirus strain Saga4 in complex with HBGA type Leb (tetraglycan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979701 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.660, 58.600, 97.170 |
| Unit cell angles | 90.00, 107.15, 90.00 |
Refinement procedure
| Resolution | 46.420 - 1.400 |
| R-factor | 0.1525 |
| Rwork | 0.151 |
| R-free | 0.18540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.202 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.420 | 1.420 | |
| High resolution limit [Å] | 1.380 | 6.170 | 1.380 |
| Rmerge | 0.075 | 0.029 | 0.930 |
| Number of reflections | 124462 | ||
| <I/σ(I)> | 12.76 | 34.72 | 1.71 |
| Completeness [%] | 99.2 | 89.5 | 96.1 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | Sodium acetate, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
