4OOW
HCV NS5B polymerase with a fragment of quercetagetin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.933400 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.600, 108.870, 134.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.900 - 2.570 |
| R-factor | 0.22063 |
| Rwork | 0.216 |
| R-free | 0.27705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hai |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.289 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.900 |
| High resolution limit [Å] | 2.570 |
| Number of reflections | 45600 |
| Completeness [%] | 94.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 291 | 0.2 M sodium acetate (pH 5.1), 16% (w/v) PEG 4000, 2 mM DTT, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
