4OOJ
Crystal structure of the N-terminal domain of the Legionella pneumophila protein SidC at 2.4A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-10-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 32 |
Unit cell lengths | 119.055, 119.055, 201.700 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.900 - 2.400 |
R-factor | 0.2031 |
Rwork | 0.200 |
R-free | 0.26180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | partial model after phasing and building in AutoSol |
RMSD bond length | 0.019 |
RMSD bond angle | 1.669 |
Data scaling software | XSCALE |
Phasing software | PHENIX (- AutoSol & PHASER - MR) |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.101 | 0.579 |
Number of reflections | 124784 | |
<I/σ(I)> | 19.03 | 2.87 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 28.65 | 28.32 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1M sodium cacodylate, 0.2M magnesium chloride, 10% PEG 1000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
2 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M sodium citrate, 0.2M sodium acetate, 10% PEG 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |