4OOI
Reduced HlyU from Vibrio cholerae N16961
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-03-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.372, 41.721, 86.981 |
| Unit cell angles | 90.00, 92.77, 90.00 |
Refinement procedure
| Resolution | 34.190 - 2.200 |
| R-factor | 0.2607 |
| Rwork | 0.258 |
| R-free | 0.30780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3jth |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.612 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.190 | 2.316 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.082 | 0.469 |
| Number of reflections | 18570 | |
| <I/σ(I)> | 8 | 2.3 |
| Completeness [%] | 97.5 | 93 |
| Redundancy | 3.78 | 3.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% PEG 8000, 0.05M potassium phosphate monobasic, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
