4OOG
Crystal structure of yeast RNase III (Rnt1p) complexed with the product of dsRNA processing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 157.972, 183.804, 61.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.719 - 2.500 |
| R-factor | 0.2168 |
| Rwork | 0.216 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3RV0 3rv1 1yyw 2nug 1o0w 3n3w 3o2r 3c4t AND 1T4O |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.777 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.650 |
| High resolution limit [Å] | 2.500 | 7.380 | 2.500 |
| Rmerge | 0.066 | 0.025 | 0.784 |
| Number of reflections | 31418 | 1329 | 4870 |
| <I/σ(I)> | 14.45 | 44.12 | 1.82 |
| Completeness [%] | 98.7 | 98.2 | 97.5 |
| Redundancy | 4.7 | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG1000, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
