4ONX
2.8 Angstrom Crystal Structure of Sensor Domain of Histidine Kinase from Clostridium perfringens.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-07-20 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.903, 82.739, 73.954 |
Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
Resolution | 29.510 - 2.800 |
R-factor | 0.19993 |
Rwork | 0.197 |
R-free | 0.24984 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.506 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.077 | 0.612 |
Number of reflections | 21394 | |
<I/σ(I)> | 31.7 | 2.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.1 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 295 | Protein: 7.5 mG/mL, 0.25 M Sodium chloride, 0.01 M Tris-HCL buffer pH 8.3; Screen: Trap96 (B9), 0.2M Ammonium sulfate, 4% (w/v) Sugar; Cryo: 1:1, screen : 50% Sucrose., VAPOR DIFFUSION, SITTING DROP, temperature 295K |