4ON3
Crystal structure of human sorting nexin 10 (SNX10)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Collection date | 2012-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 205.130, 205.130, 42.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.145 - 2.600 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.22912 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.180 |
| Data scaling software | Aimless (0.2.14) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.140 | 67.140 | 2.660 |
| High resolution limit [Å] | 2.550 | 8.830 | 2.550 |
| Rmerge | 0.136 | 0.012 | 0.012 |
| Number of reflections | 32565 | ||
| <I/σ(I)> | 7.2 | 21.7 | 1.2 |
| Completeness [%] | 98.0 | 98.2 | 95.6 |
| Redundancy | 3.1 | 3.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 4 M sodium nitrate, 0.1 M BIS-TRIS propane pH 7.0, vapor diffusion, temperature 293K |
