4ON0

Crystal Structure of NolR from Sinorhizobium fredii in complex with oligo AA DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 31
Unit cell lengths	131.306, 131.306, 68.548
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.380 - 3.000
R-factor	0.187
Rwork	0.185
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4omy
RMSD bond length	0.005
RMSD bond angle	0.861
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.400
High resolution limit [Å]	3.000
Number of reflections	23470
<I/σ(I)>	19.6
Completeness [%]	89.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	277	20% PEG-3350, 0.1 M sodium citrate/citric acid, and 0.2 M sodium citrate, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K