4OMJ
Crystal structure of SPF bound to 2,3-oxidosqualene
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.880, 73.800, 94.450 |
| Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
| Resolution | 29.890 - 1.600 |
| R-factor | 0.1556 |
| Rwork | 0.154 |
| R-free | 0.17870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o6u |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.336 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.890 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.288 |
| Number of reflections | 128890 | |
| <I/σ(I)> | 11.1 | 3.2 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 1 M Ammonium sulfate, 0.1 M Bis-Tris, 1% PEG3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
