4OM2
Crystal structure of TLE1 N-terminal Q-domain residues 1-156
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 93 |
Detector technology | CCD |
Detector | MAR CCD 130 mm |
Wavelength(s) | 1.03 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 49.586, 94.908, 130.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 53.686 - 4.000 |
R-factor | 0.2939 |
Rwork | 0.287 |
R-free | 0.34740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.783 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 76.000 |
High resolution limit [Å] | 4.000 |
Rmerge | 0.116 |
Number of reflections | 8169 |
<I/σ(I)> | 4.9 |
Completeness [%] | 100.0 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 200mM sodium thiocyanate, 15% PEG3350, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |