4OLW
Crystal structure of antibody VRC07-G54H in complex with clade A/E 93TH057 HIV-1 gp120 core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.918, 76.216, 197.186 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.416 - 2.709 |
R-factor | 0.2011 |
Rwork | 0.196 |
R-free | 0.25730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ngb |
RMSD bond length | 0.005 |
RMSD bond angle | 0.819 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.086 | 0.506 |
Number of reflections | 25978 | |
<I/σ(I)> | 14.2 | 1.8 |
Completeness [%] | 91.1 | 57.5 |
Redundancy | 3.7 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10% PEG 4000, 0.2M Sodium Acetate, 0.1M Tris 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |