4OLK

The CHAP domain of LysGH15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-28
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 62 2 2
Unit cell lengths	113.359, 113.359, 178.538
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.849 - 2.694
R-factor	0.1775
Rwork	0.174
R-free	0.20390
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	0.838
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	DM
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.690
Number of reflections	18004
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1M Bis-Tris-propane, pH 7.5, 3.8M sodium formate, VAPOR DIFFUSION, HANGING DROP, temperature 289K