Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926, 0.91837 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.157, 107.669, 68.522 |
| Unit cell angles | 90.00, 107.10, 90.00 |
Refinement procedure
| Resolution | 41.588 - 2.300 |
| R-factor | 0.2141 |
| Rwork | 0.212 |
| R-free | 0.25290 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.776 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.493 | 65.493 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.057 | 0.025 | 0.741 |
| Number of reflections | 44375 | ||
| <I/σ(I)> | 11.5 | ||
| Completeness [%] | 99.4 | 99.3 | 98.7 |
| Redundancy | 3.8 | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 16% PEG3350, 12% isopropanol, 0.1 M phenol, 0.1 M Tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 16% PEG3350, 12% isopropanol, 0.1 M phenol, 0.1 M Tris, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
