4OL0

Crystal structure of transportin-SR2, a karyopherin involved in human disease, in complex with Ran

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	74.202, 109.970, 148.462
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.184 - 2.900
R-factor	0.2244
Rwork	0.222
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.786
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.180
High resolution limit [Å]	2.900	2.900
Number of reflections	27515
Completeness [%]	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	293.15	100mM MES, 4.8% MPD, 15.2% PEG6000, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K