4OKR

Structures of Toxoplasma gondii MIC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-07-30
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	86.791, 148.052, 41.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.300 - 2.601
R-factor	0.2323
Rwork	0.230
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.734
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1565))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.300	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.268
Number of reflections	15634
Completeness [%]	99.3	63.4
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	293	0.1 M sodium acetate, 0.2 M (NH4)2SO4, 25% PEG 4000, pH 4.6, VAPOR DIFFUSION, temperature 293K