4OKO

Crystal structure of Francisella tularensis REP34 (Rapid Encystment Phenotype Protein 34 KDa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-16
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.2837
Spacegroup name	P 1
Unit cell lengths	62.712, 63.308, 81.689
Unit cell angles	70.36, 84.34, 82.53

Refinement procedure

Resolution	21.535 - 2.053
R-factor	0.2134
Rwork	0.213
R-free	0.23250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAD SE-MET SOLUTION FOR ZN-FREE CRYSTAL
RMSD bond length	0.004
RMSD bond angle	0.849
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.000	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.103	0.579
Number of reflections	68959
<I/σ(I)>	8.98	2.07
Completeness [%]	94.9	83.1
Redundancy	2.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	20% w/v PEG4000, 0.1 M sodium acetate, pH 4.7-5.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K