4OKL

Crystal Structure of d(CCCCGGTACCGGGG)2 at 1.65 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.95372
Spacegroup name	P 41 21 2
Unit cell lengths	41.270, 41.270, 87.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.048 - 1.650
R-factor	0.2284
Rwork	0.227
R-free	0.24250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v9d
RMSD bond length	0.003
RMSD bond angle	0.846
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.050	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.063	0.409
Number of reflections	9714
<I/σ(I)>	23.8	6.7
Completeness [%]	100.0	100
Redundancy	13.8	1357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1mM DNA, 50mM Sodium Cacodylate buffer, 10mM ZnCl2, 10mM Spermine, 33% MPD (v/v), pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K