4OJV
Crystal structure of unliganded yeast PDE1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 73.478, 85.151, 130.565 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.810 - 1.310 |
| R-factor | 0.17274 |
| Rwork | 0.172 |
| R-free | 0.18430 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.205 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.310 |
| Rmerge | 0.084 |
| Number of reflections | 96740 |
| <I/σ(I)> | 12.6 |
| Completeness [%] | 98.4 |
| Redundancy | 13.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | The native crystal of yPDE1 (6 mg/ml) was grown by hanging drop against a buffer of 20 mM HEPES pH 7.5, 5% PEG3350, 40 mM Li sulfate, and 12% MPD at room temperature, or a similar buffer but 15% glycerol and 20% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
