4OJI

Crystal Structure of Twister Ribozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-31
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9393
Spacegroup name	P 65 2 2
Unit cell lengths	55.380, 55.380, 175.471
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.960 - 2.300
R-factor	0.1809
Rwork	0.180
R-free	0.20280
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	MOSFLM (0.2.8)
Data scaling software	SCALA (0.2.8)
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.960
High resolution limit [Å]	2.300
Rmerge	0.079
Number of reflections	7704
<I/σ(I)>	10.2
Completeness [%]	99.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		280	PEG8000, ammonium acetate, magnesium chloride, , pH pH8, EVAPORATION, temperature 280K