4OJ5
Crystal Structure of a Putative Tailspike Protein (TSP1, orf210) from Escherichia coli O157:H7 Bacteriohage CBA120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 123.621, 147.823, 170.631 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.438 - 1.800 |
| R-factor | 0.1867 |
| Rwork | 0.187 |
| R-free | 0.20330 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | SeMet Tailspike protein structure |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.423 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.900 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.094 | 0.510 |
| Number of reflections | 287155 | |
| <I/σ(I)> | 11.9 | 2.2 |
| Completeness [%] | 99.8 | 99.6 |
| Redundancy | 6.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.1 M Tris pH 7.0-7.6, 20% PEG-1000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
