4OJ2
The structure of aquaporin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 95.717, 95.717, 79.058 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.070 - 3.050 |
| R-factor | 0.25893 |
| Rwork | 0.257 |
| R-free | 0.29897 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.693 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.070 | 3.290 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 7153 | |
| <I/σ(I)> | 38.8 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 30% PEG 400, 0.1M sodium chloride, 0.1M MOPS, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |
