4OJ0

mCardinal V218E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-03
Detector	ADSC QUANTUM 315
Wavelength(s)	1.115834
Spacegroup name	P 21 21 21
Unit cell lengths	72.550, 102.942, 116.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.437 - 1.700
R-factor	0.1652
Rwork	0.165
R-free	0.19150
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.464
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.437
High resolution limit [Å]	1.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	285	0.2 M Calcium Acetate, 0 .1 M MES pH 6.0, 20% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 285K