4OIH

Importin Alpha in Complex with the Bipartite NLS of Prp20

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.954946
Spacegroup name	P 21 21 21
Unit cell lengths	78.910, 89.920, 99.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.370 - 2.100
R-factor	0.16941
Rwork	0.168
R-free	0.20125
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ul1
RMSD bond length	0.022
RMSD bond angle	2.081
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.370	2.154
High resolution limit [Å]	2.100	2.100
Number of reflections	53834
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296.15	500mM sodium citrate, 10mM DTT, pH 6.0-8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296.15K