4OHC
Crystal structure of orotate phosphoribosyltransferase (OPRTase) from Burkholderia cenocepacia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-11 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.310, 82.970, 83.100 |
| Unit cell angles | 105.83, 104.27, 117.36 |
Refinement procedure
| Resolution | 50.000 - 1.850 |
| R-factor | 0.15758 |
| Rwork | 0.156 |
| R-free | 0.19659 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m3h |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.573 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.900 | |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.502 | |
| Number of reflections | 130679 | |
| <I/σ(I)> | 17.9 | 2.75 |
| Completeness [%] | 97.4 | 95.8 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | JCSG+ g6: 200mM sodium malonate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
