4OGK
X-ray structure of the uridine phosphorylase from Salmonella typhimurium in complex with thymidine at 2.40 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-11-04 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 61 |
| Unit cell lengths | 89.970, 89.970, 256.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.993 - 2.400 |
| R-factor | 0.2228 |
| Rwork | 0.220 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ip0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.059 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | |
| High resolution limit [Å] | 2.400 | 6.810 | |
| Rmerge | 0.077 | ||
| Number of reflections | 45188 | 2493 | 13469 |
| <I/σ(I)> | 16.33 | 45.19 | |
| Completeness [%] | 99.6 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 294 | 40% (w/v) polyethylene glycol 3350, 0.1 M Tris-maleate-NaOH, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
