4OGE
Crystal structure of the Type II-C Cas9 enzyme from Actinomyces naeslundii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.415, 133.025, 80.690 |
| Unit cell angles | 90.00, 96.22, 90.00 |
Refinement procedure
| Resolution | 68.692 - 2.201 |
| R-factor | 0.1883 |
| Rwork | 0.186 |
| R-free | 0.22620 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.139 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.200 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 78425 | |
| Completeness [%] | 98.0 | 86.8 |
| Redundancy | 8.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% (w/v) PEG 8000, 0.25 M calcium acetate, 50 mM magnesium acetate and 5 mM spermidine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
