4OG2
The crystal structure of a solute-binding protein (N280D mutant) from Anabaena variabilis ATCC 29413 in complex with leucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.326, 101.124, 149.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.498 - 1.099 |
| R-factor | 0.1303 |
| Rwork | 0.129 |
| R-free | 0.14970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nqr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.112 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.500 | 1.120 |
| High resolution limit [Å] | 1.099 | 1.100 |
| Rmerge | 0.074 | 0.620 |
| Number of reflections | 296681 | |
| <I/σ(I)> | 25.1 | 1.53 |
| Completeness [%] | 98.8 | 91 |
| Redundancy | 5.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Magnesium Chloride, 0.1 M Tris:HCl, 30% (v/v) PEG 400, 10mM Leucine, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
