4OFY

Crystal Structure of the Complex of SYG-1 D1-D2 and SYG-2 D1-D4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-05
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0088
Spacegroup name	C 1 2 1
Unit cell lengths	286.786, 116.144, 98.032
Unit cell angles	90.00, 107.62, 90.00

Refinement procedure

Resolution	41.061 - 3.300
R-factor	0.2619
Rwork	0.259
R-free	0.30590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB Entries 4OF3 4ofp
RMSD bond length	0.004
RMSD bond angle	0.904
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1593))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	44580
<I/σ(I)>	13.4
Completeness [%]	95.9
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	295	16% PEG 3,350, 0.2 M Triammonium citrate, 0.1 M MES pH 6.4, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K