4OFF
Crystal structure of apo carboxy cGMP binding domain of Plasmodium falciparum PKG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2011-12-14 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 25.781, 57.683, 82.780 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.236 - 1.888 |
R-factor | 0.1798 |
Rwork | 0.177 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.015 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.630 |
High resolution limit [Å] | 1.888 |
Rmerge | 0.088 |
Number of reflections | 10442 |
<I/σ(I)> | 18.2 |
Completeness [%] | 99.6 |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 295.1 | 0.2 M lithium sulfate, 25 % (w/v) PEG 3350 and 0.1 M Bis-Tris pH 5.5. , VAPOR DIFFUSION, temperature 295.1K |