4OF6

Crystal Structure of SYG-1 D1, Crystal form 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 42 21 2
Unit cell lengths	84.809, 84.809, 74.129
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.623 - 1.696
R-factor	0.1763
Rwork	0.175
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4of0
RMSD bond length	0.011
RMSD bond angle	1.327
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1160))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.696
Number of reflections	30345
<I/σ(I)>	32.8
Completeness [%]	99.5
Redundancy	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	1.2 M Trisodium citrate, 0.1 M HEPES pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K