4OF3

Crystal Structure of SYG-1 D1-D2, Glycosylated

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 32 2 1
Unit cell lengths	92.648, 92.648, 129.014
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.599 - 2.500
R-factor	0.1992
Rwork	0.196
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4of0
RMSD bond length	0.007
RMSD bond angle	1.076
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	22777
<I/σ(I)>	25.1
Completeness [%]	99.6
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	25% PEG 200, 5% PEG 3,000, 0.1 M MES pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K