4OEH
X-ray Structure of Uridine Phosphorylase from Vibrio cholerae Complexed with Uracil at 1.91 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.255, 71.763, 89.321 |
| Unit cell angles | 69.14, 72.15, 85.79 |
Refinement procedure
| Resolution | 44.278 - 1.910 |
| R-factor | 0.1744 |
| Rwork | 0.172 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h1t |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.154 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 79.600 | 44.278 | 2.010 |
| High resolution limit [Å] | 1.907 | 6.030 | 1.910 |
| Rmerge | 0.020 | 0.409 | |
| Total number of observations | 6028 | 27074 | |
| Number of reflections | 99133 | ||
| <I/σ(I)> | 9 | 33.4 | 1.9 |
| Completeness [%] | 94.4 | 95.7 | 92.8 |
| Redundancy | 1.8 | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | Magnesium chloride hexahydrate, TRIS hydrochloride, PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
