4ODX

4E10 germline encoded precursor no.7 in complex with epitope scaffold T117

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2012-07-27
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	72.912, 78.004, 78.396
Unit cell angles	90.00, 112.29, 90.00

Refinement procedure

Resolution	62.730 - 3.100
R-factor	0.28106
Rwork	0.280
R-free	0.30433
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.865
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.730	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	13982
Completeness [%]	98.5	97.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	10% w/w PEG 10,000, 0.1 M NH4)Ac, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K