4ODW
Unliganded Fab structure of lipid A-specific antibody A6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.129, 69.235, 69.066 |
| Unit cell angles | 70.99, 72.39, 88.29 |
Refinement procedure
| Resolution | 65.270 - 2.720 |
| R-factor | 0.2325 |
| Rwork | 0.231 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4odv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.613 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 65.270 | 35.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.810 | 2.700 |
| Rmerge | 0.040 | 0.031 | 0.472 |
| Number of reflections | 18489 | ||
| <I/σ(I)> | 15.4 | ||
| Completeness [%] | 98.1 | 97.1 | 98.3 |
| Redundancy | 2 | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris-HCl, 35% (w/v) PEG 1000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
