4ODU
Unliganded Fab structure of lipid A-specific antibody S1-15
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.787, 77.638, 71.958 |
| Unit cell angles | 90.00, 96.83, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.290 |
| R-factor | 0.19 |
| Rwork | 0.187 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4odt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.418 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.063 | 0.615 |
| Number of reflections | 37458 | |
| <I/σ(I)> | 11.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 289 | 25% (w/v) PEG 2000 MME, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
