4ODT
Fab Structure of lipid A-specific antibody S1-15 in complex with lipid A carbohydrate backbone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.471, 77.471, 156.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.400 - 1.950 |
| R-factor | 0.199 |
| Rwork | 0.197 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ods |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.331 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.5) |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.400 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.088 | 0.786 |
| Number of reflections | 35405 | |
| <I/σ(I)> | 10.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 15.7 | 15.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 289 | 0.1M NaCl, 0.1M Bicine, and 30% (w/v) PEG 550 MME, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
