4ODK
Structure of SlyD from Thermus thermophilus in complex with T1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9191 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.500, 52.990, 48.910 |
Unit cell angles | 90.00, 111.18, 90.00 |
Refinement procedure
Resolution | 29.096 - 1.401 |
R-factor | 0.1403 |
Rwork | 0.139 |
R-free | 0.16710 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.027 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.100 | 1.440 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 40188 | |
<I/σ(I)> | 23.03 | 2.02 |
Completeness [%] | 97.6 | 93.7 |
Redundancy | 3.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 13.2% PEG1500, 0.1 M HEPES, pH 7.5, 0.05 M sodium chloride, 11% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |