4ODH
Crystal structure of human Fab CAP256-VRC26.UCA, a potent V1V2-directed HIV-1 neutralizing antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.456, 81.194, 69.193 |
| Unit cell angles | 90.00, 123.95, 90.00 |
Refinement procedure
| Resolution | 33.144 - 2.894 |
| R-factor | 0.2251 |
| Rwork | 0.224 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u2s |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.829 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.894 | 6.240 | 2.894 |
| Rmerge | 0.110 | 0.368 | |
| Number of reflections | 7503 | ||
| <I/σ(I)> | 5.9 | 1.8 | |
| Completeness [%] | 85.7 | 81.4 | 82.8 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 27% PEG8000, 0.1 M HEPES, pH 7.5, cryoprotectant: 20% PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
