4OD5
Substrate-bound structure of a UbiA homolog from Aeropyrum pernix K1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2013-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.104, 122.829, 423.416 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.735 - 3.557 |
| R-factor | 0.2805 |
| Rwork | 0.279 |
| R-free | 0.30140 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.809 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.557 |
| Rmerge | 0.135 |
| Number of reflections | 44997 |
| <I/σ(I)> | 10 |
| Completeness [%] | 99.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 295 | 0.1M Na2SO4, 0.1M sodium cacodylate pH 5.0, 26.5% (w/v) PEG400, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
