4OCM

Crystal Structure of the Rpn8-Rpn11 MPN domain heterodimer, crystal form Ib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00000
Spacegroup name	P 1 2 1
Unit cell lengths	63.401, 44.970, 200.043
Unit cell angles	90.00, 98.40, 90.00

Refinement procedure

Resolution	30.000 - 1.990
R-factor	0.2181
Rwork	0.216
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ocl
RMSD bond length	0.022
RMSD bond angle	1.933
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	197.898	44.970	2.100
High resolution limit [Å]	1.990	6.290	1.990
Rmerge	0.078	0.030	0.600
Total number of observations		7717	31869
Number of reflections	74800
<I/σ(I)>	9.1	16.4	1.3
Completeness [%]	96.8	97.9	88
Redundancy	3.2	3	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROPS	6	291	50 mM MES, pH 6.0, 200 mM calcium acetate, 22% PEG3350, VAPOR DIFFUSION, HANGING DROPS, temperature 291K