4OC9
2.35 Angstrom resolution crystal structure of putative O-acetylhomoserine (thiol)-lyase (metY) from Campylobacter jejuni subsp. jejuni NCTC 11168 with N'-Pyridoxyl-Lysine-5'-Monophosphate at position 205
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.434, 149.787, 186.591 |
| Unit cell angles | 100.58, 92.47, 90.10 |
Refinement procedure
| Resolution | 29.650 - 2.350 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.009 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.113 | 0.448 |
| Number of reflections | 255734 | |
| <I/σ(I)> | 10.9 | 2.7 |
| Completeness [%] | 94.9 | 95.6 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 295 | protein: 1.85 mg/mL crystallization: 25%PEG 3350, 0.2M Mg Chloride, 0.1M Tris, cryo conditions: paraton, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
