4OBU
Ruminococcus gnavus tryptophan decarboxylase RUMGNA_01526 (apo)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1159 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.630, 145.770, 165.070 |
| Unit cell angles | 72.85, 88.84, 88.30 |
Refinement procedure
| Resolution | 79.369 - 2.804 |
| R-factor | 0.2324 |
| Rwork | 0.231 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.670 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.370 | 2.905 |
| High resolution limit [Å] | 2.804 | 2.804 |
| Number of reflections | 121043 | |
| Completeness [%] | 94.7 | 96.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1M Bicine pH 8.5, 25% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
