4OBS
Crystal structure of human alpha-L-iduronidase in the P212121 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2013-06-04 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97939 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.640, 78.030, 112.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.070 - 2.260 |
| R-factor | 0.19424 |
| Rwork | 0.192 |
| R-free | 0.23522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mj2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.094 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.070 | 2.319 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 30055 | |
| <I/σ(I)> | 31.3 | 8.35 |
| Completeness [%] | 98.1 | 85 |
| Redundancy | 9.65 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.18 M sodium potassium tartrate, 18% PEG3350, 50 mM ammonium sulfate, 10 mM HEPES, pH 7.5, 3% MPD, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
