4OBR
Crystal structure of human alpha-L-iduronidase complex with alpha-L-iduronic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97940 |
| Spacegroup name | H 3 |
| Unit cell lengths | 258.940, 258.940, 71.530 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.940 - 2.460 |
| R-factor | 0.20418 |
| Rwork | 0.202 |
| R-free | 0.23929 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mj2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.940 | 2.524 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Number of reflections | 63496 | |
| <I/σ(I)> | 12.89 | 3.43 |
| Completeness [%] | 97.8 | 82.8 |
| Redundancy | 3.6 | 2.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.01 M HEPES, pH 7.5, 0.26 M sodium potassium tartrate, 20% PEG3350, 50 mM ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
