4OB4

Structure of the S. venezulae BldD DNA-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 61 2 2
Unit cell lengths	79.970, 79.970, 117.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.256 - 2.800
R-factor	0.2363
Rwork	0.230
R-free	0.29550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ewt
RMSD bond length	0.010
RMSD bond angle	1.165
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	69.256
High resolution limit [Å]	2.800
Rmerge	0.113
Number of reflections	5897
<I/σ(I)>	12.4
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	30% PEG 400, 0.1 M MgCl2, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K