4OAY
BldD CTD-c-di-GMP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-01 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 32 |
| Unit cell lengths | 86.500, 86.500, 151.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 74.910 - 1.950 |
| R-factor | 0.208 |
| Rwork | 0.198 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | P32 STARTING MODEL WITH SEMET |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.512 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.700 | 2.060 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.057 | 0.395 |
| Number of reflections | 90983 | |
| <I/σ(I)> | 11.4 | 1.7 |
| Completeness [%] | 98.5 | 62.2 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 298 | 35% PEG 400, 0.1 M acetic acid pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
