4OAW
Fab structure of anti-HIV gp120 V2 mAb 2158
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-11-27 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 32 |
| Unit cell lengths | 73.710, 73.710, 215.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 54.831 - 2.800 |
| R-factor | 0.1859 |
| Rwork | 0.182 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qad |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.338 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 215.000 | 2.800 |
| High resolution limit [Å] | 2.800 | 2.760 |
| Number of reflections | 31988 | |
| <I/σ(I)> | 5.6 | 1.8 |
| Completeness [%] | 98.2 | 98.5 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 23.5% PEG 4K/0.17M ammonium sulfate/15% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
