4OAR
Progesterone receptor with bound ulipristal acetate and a peptide from the co-repressor SMRT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 78 |
| Detector technology | CCD |
| Collection date | 2008-06-13 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.180, 87.180, 90.910 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.590 - 2.410 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ovh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.500 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.590 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.089 | 0.399 |
| Number of reflections | 13598 | |
| <I/σ(I)> | 16 | 3.05 |
| Completeness [%] | 84.8 | 67.8 |
| Redundancy | 6.65 | 2.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 15% PEG3350, Tris,HCl 50mM, NaCl 400mM, 10% Glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
